While there are many molecules with antibacterial activity, to develop new antibiotics it is necessary to identify the targets, molecules essential for bacterial life to which the active molecules bind, causing bacterial death. To address this bottleneck, we have generated mutant libraries that represent the genome of Burkholderia cenocepacia, including essential genes.
We use these libraries to quantify mutant response during exposure of novel molecules with antibiotic activity to help identify the mechanism of action of the new compounds. To track the mutants growing together as a pool, we use next generation sequencing techniques. Once the mechanism of action of a new antibiotic is determined structure-activity relationship (SAR) studies can start to improve antibiotic activity
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